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ملخص البحث |
Abstract: The present study was designed to screen in-vitro antibacterial activity of Paronychia argentea Lam., Matricaria aurea , and Verbascum sinuatum L from Gaza strip-Palestine. The dried aerial parts of plant were successively extracted with chloroform, ethanol and aqueous solvents using serial exhaustive cold maceration extraction. All extracts were screened for its antibacterial activity using agar well diffusion method and micro-dilution technique. The microorganisms used for antibacterial activity were Escherichia coli, Klebsiella pneumoniae, Morganella morganii, Methicillin-sensitive Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus. The average diameter of inhibition zones against the tested bacteria ranged from 9 to 14 mm, 9 to 19 mm and 9 to 20 mm for chloroform, ethanol and aqueous extract, respectively. The extracts showed antibacterial activity were subjected to minimum inhibitory concentration assay; a micro-broth dilution assay was performed on 96-well plates using 2, 3, 5-Triphenyl Tetrazolium Chloride (TTC) as an indicator for bacterial growth, the average minimum inhibitory concentrations (MICs) values ranged from 2.08 to 33.33 mg/ml, 1.04 to 8.33 mg/ml and 0.52 to 4.17 mg/ml for chloroform, ethanol and water extracts, respectively. Phytochemical analysis revealed that Paronychia argentea, Matricaria aurea chloroform extracts appear to contain sterols, while, essential oils were found to be present in all the three plant extracts what might indicate lipophilic character. Tannins, saponins and flavonoids were found to be present in all the three plant extracts. Test for carbohydrates were positive for the three plant aqueous extracts. None of the extracts tested were positive for alkaloids. |
