The aim of this study is to identify the biochemical changes following oral administration of lead acetate at a dose of 40 mg/kg body wt. daily for different intervals (1 day, 3 days, 5 days, 10 days and 20 days) and treatment by Nigella sativa oil or olive oil or garlic lobes were evaluated in adult male domesticated rabbits.  Lead acetate  administration revealed that levels of serum albumin , total proteins, lactate dehydrogenase (LDH) activity, aspartate aminotransferase (AST) and acid phosphatase were affected due to lead toxicity. In general, results indicated a general decrease in globulin concentration and alanin aminotransferase (ALT) activity under the influence of lead acetate administration at the different intervals of the experiment. However, alkaline phosphatase activity in rabbits blood serum generally increased due to lead acetate administration. Signs of improvements in the previous biochemical parameters were noticed after oral administration of Nigella sativa oil or olive oil and/or garlic lobes during lead acetate administration.